Which reference genome should the designs target?
Indices are pre-built for each organism's individual gene sequences
Enter one or more
FASTA format sequences to evaluate e.g:

>some sequence
ATATCGATGCTAGCTAGCT
The file should contain
FASTA format sequences to evaluate e.g:

>some sequence
ATATCGATGCTAGCTAGCT
Should the CRISPR designs should be checked for any secondary off-target effects.
This means if they will cut in any exogenous, foreign introduced sequences.
Select individual off-targets from the list or paste a set of fasta sequences into the text area
This text area only accepts FASTA format sequences as input e.g.:
>some sequence
ATATCGATGCTAGCTAGCT
GATCGTAGCTAGCTAGCTA
E-CRISP-TEST
This page is old and discontinoued.
plase use www.e-crisp.org for research
DKFZ
De-novo Evaluation Help Links

1. Select organism:

[HELP]

Number of 5' mismatch positions ignored by the programm.

Tolerated edit distance to the target sequence


2. Enter target sequence:



FASTA example [HELP]
Clear

Please enter the target sequence without the PAM. In FASTA a format e.g:

>some sequence
ATATCGATGCTAGCTAGCT.
All possible PAMs will be added during the search process.

Alternatively upload a file in FASTA format or a new line separated list of gene symbols and/or accesion numbers

[HELP]


The older version of E-CRISP can be reached Here



Boutros lab, E-CRISP-Version 4.2
For suggestions please contact us at crispr@dkfz.de